Bioimaging Platform

 

 

 

 

 

The LCSB Bioimaging Platform provides access to and support for a broad palette of advanced microscopy and flow cytometry instruments, enabling researchers to perform studies from the tissue level and down to sub-cellular scales.

The focus of the Bioimaging Platform is to grant researchers access to state-of-the-art analytical imaging technologies. As such, the platform comprises of advanced microscopy systems for high resolution studies of fixed and live samples as well as for low-phototoxicity imaging of whole organs and organisms. Image analysis is supported by strong in-house capacities and the university’s high-performance computing facility.

We work in close collaboration with platform users and research groups to support instrument use, quality control, data acquisition, analysis, and interpretation. Specific expertise in image analysis enables us to assist users with the development of custom-made software tools. The on-going strategic development of the platform is also organized in strong interactions with the users and their needs.

Equipment

The Bioimaging platform currently includes seven advanced microscopy systems, two flow cytometers, as well as several high-performance analysis workstations. Task of the platform is to train users to become independent in operating the instruments, including basic troubleshooting and maintenance. All instruments of the platform are made available to research groups within the LCSB and through collaborations to University of Luxembourg internal and external partners.

Microscopy Systems

High content screening system (Perkin Elmer Opera)

  • four laser lines (405, 488, 561, and 640 nm)
  • four objectives available (10x air, 20x water, 40x water, 60x water)
  • automated sample manipulation capacities
  • full environmental control (CO2 , humidity and temperature)
  • dual camera system

 

High content screening system (Yokogawa CV8000)

  • five laser lines (405, 445, 488, 561, and 640 nm)
  • six objectives available (4x air, 10x air, 20x air, 20x water, 40x water, 60x water, 20x air LWD)
  • automated liquid dispenser for 96- and 384-well plates
  • full environmental control (CO2 , humidity and temperature)
  • quadruple camera system

Confocal microscope (Zeiss LSM 710)

  • six laser lines (405, 458, 488, 514, 561, and 633 nm)
  • four objectives available (5x air, 20x air, 40x oil, and 63x oil)
  • two ultrasensitive gallium arsenide phosphide detectors

 

Spinning disk confocal microscope (Zeiss Cell Observer)

  • five laser lines (405, 488, 515, 561, and 640 nm)
  • four objectives available (10x air, 25x water & glycerol & oil DIC, 40x oil DIC UV-VIS-IR, 63x oil DIC)
  • full environmental control (CO2 , humidity and temperature)

 

Laser microdissection microscope (Zeiss PALM)

  • high-power xenon light source
  • CapMover II sample collector
  • automated high precision stage
  • dual camera system
  • three objectives (5x air, 20x air PlasDIC, 40x air PlasDIC)

 

Inverted fluorescence microscope with confocal scanner (Nikon Eclipse Ti-E RCM)

  • high-speed monochromator-based xenon arc light source
  • customizable triple LED light source
  • seven objectives available (2x air, 10x air, 20x air, 40x air, 40x oil, 60x oil, and 100x oil)
  • full environmental control (CO2 , humidity and temperature)
  • Confocal.nl RCM laser scanner with four laser lines (405, 488, 561, and 633 nm) and optical bypass

 

Selective plane illumination microscope (LCSB-SPIM)

  • constructed in-house and under ongoing development
  • four laser lines (405, 488, 561, and 633 nm)
  • three objectives available (20x water, 25x water with ultra-long working distance, 40x water)
  • high-precision 4D stage

 

Super-resolution microscope (Leica TCS SP8 STED 3X FALCON)

  • supercontinuum white-light laser
  • four spectrally adjustable detector channels (two multi-alkali PMTs and two highly sensitive HyDs)
  • six objectives available (10x air, 20x water & glycerol & oil, 40x air, 63x oil, 93x STED glycerol, and 100x STED oil)
  • full environmental control (CO2 , humidity and temperature)

 

Flow Cytometers

Fluorescence activated cell sorter (Becton Dickinson FACSAriaIII)

  • four laser lines (405, 488, 561, and 633 nm)
  • 4-way aseptic sorting, automatic cell deposition unit allowing to sort into microwell plates and arbitrary supports
  • HEPA filter for aerosol control
  • the ability to analyse and sort based on up to 15 parameters simultaneously (13 fluorescence, 2 geometric scatter)

 

Cell analyser (Becton Dickinson LSRFortessa)

  • four laser lines (405, 488, 561, and 640 nm)
  • a high-throughput sampling unit for 96- and 384-well plates
  • the ability to analyse up to 15 parameters simultaneously (13 fluorescence, 2 geometric scatter)

 Fluorescence activated cell sorter (Becton Dickinson FACSMelody)

  • three laser lines (405, 488, and 561 nm)
  • 4-way aseptic sorting, automatic cell deposition unit allowing to sort into microwell plates and arbitrary supports
  • biosafety cabinet for sample protection and aerosol control
  • ability to sort based on up to 10 parameters simultaneously (8 fluorescence, 2 geometric scatter)

 

 

Contact information

Reach Us
E-mail: LCSB-bioimaging [at] uni.lu
Phone: (+352) 466 644 6723

Visiting Address
Bioimaging Platform
BioTech II - Luxembourg Centre for Systems Biomedicine
Campus Belval, Université du Luxembourg
6, Avenue du Swing - L-4367 Belvaux
Location on Google Maps ⇗

Platform Manager

 

 

 

 

Paul Antony

 

 

 

 

 

Technicians

 

 

 

 

Olga Kondratyeva

 

 
URL:  https://wwwen.uni.lu/lcsb/services/platforms/bioimaging_platform Date:  Wednesday, 31 May 2023, 04:58