A new fascinating workshop in Biology will join our recent addition in mathematics and will be offered on a regular basis during the school year 2018/2019! In this practical workshop entitled – The enzyme Dld3 shows its colours – the participants will explore the world of enzymes and discover specific methods required for the characterization of proteins.
Proteins and enzymatic reactions
At the beginning of the workshop, students are introduced to the scientific background and learn more about the enzyme Dld3. This enzyme was first characterised in the research group of Dr Carole Linster at the Luxembourg Centre for Systems Biomedicine of the University of Luxembourg. Thus, the workshop is thematically and also methodically very closely linked to current research. After the presentation, the participants analyse the enzyme themselves in the lab. The practical work is accompanied by brief explanations about the different experiments. Essential practical elements performed in the lab are the extraction and purification of proteins from wild type and genetically engineered Escherichia coli bacteria and yeast cells via specific column systems. Furthermore, the enzyme is detected by a Coomassie-stained protein gel and by a substrate-specificity assay. Finally, a joint discussion about the results will finish the day in the lab.
A successful first test run!
At the beginning of June, 9 students of a 2eC from the École Privée Fieldgen were the first ones to discover the new workshop during their second visit here to the Scienteens Lab. This first test run was very well received by the students and the Scienteens Lab team is looking forward to offering this course to all classes starting from school year 2018/2019!
Our one-day workshop “DNA on the tanning bed” can of course still be booked. To know more, go to the webpage dedicated to the biology workshops and book a workshop via Appointy.
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Ref. : Saccharomyces cerevisiae Forms d-2-Hydroxyglutarate and Couples Its Degradation to d-Lactate Formation via a Cytosolic Transhydrogenase, Journal of Biological Chemistry, Becker-Kettern et al., 2016.