Light Microscopy Facility

The Light Microscopy Facility was established in 2007 to support Life Science Research at the University of Luxembourg. Its open access to University members as well as to external users from academia and industry is promoting interactions across a wide range of projects and disciplines and is adding in return to its expertise. The vision of this facility is not only focused on the provision of equipment, but also on the introduction of users, interested scientists and students into the fundamental principles and advanced applications of light microscopy. This is an indispensable prerequisite for the understanding of this technology and for the recognition of its potential impact on individual research projects.

Services provided

  • Provision of equipment
  • Hands-on training for microscopy & microinjection
  • Hands-on workshops
  • Lectures / seminars
  • Image processing support
  • Project / experiment planning support
  • Relevant technical support for the preparation grant applications
  • Sample preparation support
  • 3D printing

Collaboration partners

  • Andor Technology, Belfast, Ireland
  • Eppendorf AG, Hamburg, Germany
  • Evrogen, Moskow, Russia
  • Nikon BELUX, Brussels, Belgium
  • ZEISS Microscopy Labs, Munich, Germany

 

 

 

Contact

Dr. Andreas Girod

  • Coordinator Light Microscopy Facility
  • Principal investigator
  • Life Sciences Research Unit-FSTC
  • Office: E1-104B
  • Microscope room: E3-305A
  • Campus Belval, House of Biomedicine / Biotech I (BT1)
  • 7, avenue des Hauts-Fourneaux
    L-4362 Esch-sur-Alzette
  • Phone office: +352 46 66 44 6863
  • E-mail: andreas.girod@uni.lu

 

Equipment

Andor Revolution Spinning Disk Confocal Microscope (this equipment was funded by the Fondation Cancer)

Main Specifications:-Nikon Ti Microscope-Lumencor SPECTRA X Light Engine (high performance LED light source for fast, multi-colour wide-field imaging) -Yokogawa W1 Scan Head (spinning disk confocal scanner)-Andor Laser Combiner (405nm, 488nm, 561nm, 640nm & 660nm Laser)-Nikon TIRF-M Module (Total Internal Reflection Fluorescence Microscopy)-Till Photonics FRAP-PA Module (Point Scanner for photo-manipulation like bleaching, photo-activation)-Andor iXon 888 EM-CCD camera (1024x1024 pixel )-Andor Zyla scmos camera

Zeiss LSM510 Meta Confocal Microscope

Along the standard configuration, the system will (soon) be equipped with 

  • a high performance LED light source for fast, multi-colour wide-field imaging
  • a Yokogawa CSU10 confocal scan head ( spinning disk confocal scanner)
  • a PCO FLIM camera (Fluorescence Lifetime Imaging Microscopy)
  • 4 color laser combiner for CSU10 and the FLIM camera

The additional modules will be controlled via Andor iQ3 software
 

Eppendorf Semi-Automatic Microinjection System

Leica Micro-dissection System

Workstation for image processing

Deconvolution - Visualization - Analysis

https://svi.nl

OpenSPIM system (in collaboration with LCSB)

(SPIM: Selective Plane Illumination Microscopy, Light Sheet Microscopy) 405nm, 488nm, 561nm & 640nm laser combiner

LabView based software

3D Printer Ultimaker S5

Ultimaker Cura software

Rhinoceros 3D modeling software

Workshops & Conferences

  • 06.2012: Workshop on microinjection
  • 05.11.2012: Introduction into confocal microscopy. Invited speaker: Dr. Sven Terclavers, Carl Zeiss nv-sa, Belgium
  • 14-18.01.2013: International Hands-On Light Microscopy Workshop – Basic & Advanced Techniques (FNR RESCOM funded; about 40 participants)
  • 20-24.02.2017: The 2nd edition of the international Hands-on Light Microscopy Workshop (about 70 participants). https://holm2017.uni.lu
  • 29.09.2017: Huygens Workshop on Deconvolution - Visualization - Analysis of image data
  • 02-05.02.2019: Neubias Training School for Bioimage Analysis (EU COST Action, about 100 participants)
  • 05-08.02.2019: Neubias Bioimage Analysis Symposium (EU COST Action, FNR RESCOM funded; almost 300 participants)

Other Activities:

  • National representative for the Neubias COST Action
  • Member of the European Light Microscopy Initiative
  • Stakeholder of Euro-Bioimaging
  • Lectures in master courses (Luxembourg & Greece)

Rules & regulations

Relevant Links

Past and current Grants & Projects

 01.2013 - 12.2014     

  • Fondation Cancer
  • Project title: 'Implémentation d’une plate de microscopie en temps réel pour l’étude des mécanismes moléculaires associés aux cancers dans le contexte de la cellule vivante.'
  • Budget: 410,205€

01.2013                                 

  • FNR RESCOM Exploratory Workshops
  • Title: Hands-on light microscopy workshop- basic and advanced techniques
  • Funding: 9.132€

07.2014 - 09.2017      

  • FNR CORE Project
  • Title: Investigation of the regulatory pathways that govern fidelity of cell division tumorigenesis aneuploidy
  • Budget: 597.000

01.2015 - 06.2017      

  • University internal research project
  • Title: A journey of chromatin remodeling factors from chromatin to spindle and back regulation function
  • Budget: 218.000€

01.2018 - 06.2021      

  • Fondation Cancer Project
  • Mapping the interaction profile of microtubule associated proteins (MAPs) in time and space, during out of control cell division in cancer –“2MAP cancer”
  • Budget: 533.868€

02.2019                      

  • FNR RESCOM Scientific Conference
  • Title: Hands-on light microscopy workshop- basic and advanced techniques